中国水稻科学

中国水稻科学 ›› 2012, Vol. 26 ›› Issue (1): 1-4.DOI: 10.3969/j.issn.10017216.2012.01.001

• 研究报告 •    下一篇

水稻短根毛突变体ksrh1的遗传分析和基因定位

丁沃娜,吴晶,罗丽丽,钱周婷,朱世华   

  1. 宁波大学  植物分子生物学研究室, 浙江 宁波 315211
  • 收稿日期:2011-03-25 修回日期:2011-05-18 出版日期:2012-01-10 发布日期:2012-01-10
  • 基金资助:

    国家自然科学基金资助项目 (31071388)； 宁波市自然科学基金资助项目（2008A610070）。

Genetic Analysis and Gene Mapping of a Short Root Hair Mutant ksrh1 in Rice

DING  Wona, WU Jing, LUO Lili, QIAN Zhouting, ZHU Shihua   

  1. Laboratory of Plant Molecular Biology,  Ningbo University,  Ningbo 315211,  China
  • Received:2011-03-25 Revised:2011-05-18 Online:2012-01-10 Published:2012-01-10

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摘要： 从EMS诱变的籼稻品种Kasalath突变体库中筛选获得了一个短根毛突变体，命名为ksrh1。该突变体在苗期表现为根毛变短，除此之外其表型与野生型没有显著差异。遗传分析表明，该突变性状受1个隐性单基因控制。将突变体ksrh1与粳稻品种日本晴杂交构建F2定位群体，利用已公布的水稻SSR标记和自行设计的STS标记对突变位点进行基因定位，最终将KSRH1定位在水稻第1染色体长臂上的S3578和S3584之间，物理距离约为67 kb。

关键词: 水稻, 短根毛突变体； 遗传分析； 基因定位

Abstract: A rice mutant with significantly shorter root hairs was isolated from an EMSinduced mutant library of  indica Kasalath.  The mutant was named as Kasalath short root hair 1 (ksrh1). Except shorter root hairs, there was no significant difference between ksrh1 and its wild type. Genetic analysis showed that the mutant was controlled by a single recessive nuclear gene. To map  the gene  KSRH1, the mutant  ksrh1 and Nipponbare were crossed to generate an F2 population. By using the published SSR markers and some newly designed STS markers,  KSRH1   was mapped to a 67 kb region between the STS markers S3578 and S3584 on the long arm of chromosome 1.

Key words: rice (Oryza sativa L.), short root hair mutant, genetic analysis, gene mapping

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